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Image Search Results
Journal: Molecular Therapy. Methods & Clinical Development
Article Title: Silencing p75 NTR prevents proNGF-induced endothelial cell death and development of acellular capillaries in rat retina
doi: 10.1038/mtm.2015.13
Figure Lengend Snippet: Silencing p75 NTR suppresses proNGF-induced vascular cell death and apoptotic marker in rat retina. Representative images of trypsin-digested retinas stained with TUNEL (green) and counterstained with DAPI (blue) are shown for ( a ) GFP+Scr, ( b ) GFP+p75shRNA, ( c ) proNGF+Scr, and ( d ) proNGF+p75shRNA. TUNEL assay showed several positive cells in proNGF-overexpressing retinas, suggesting that capillary degradation occurs through apoptosis. ( e ) Representative western blots and statistical analysis of pJNK and JNK and ( f ) cleaved-PARP normalized to actin. 2 × 2 analysis showed interaction between proNGF expression and silencing p75 NTR . Overexpression of proNGF induced significant activation of JNK and cleavage of PARP compared with GFP-controls. These effects were mitigated by p75shRNA but not scrambled. Results presented as mean ± SD. n = 4–6 per group. * P < 0.05 versus other groups.
Article Snippet: For cell viability experiments,
Techniques: Marker, Staining, TUNEL Assay, Western Blot, Expressing, Over Expression, Activation Assay
Journal: Molecular Therapy. Methods & Clinical Development
Article Title: Silencing p75 NTR prevents proNGF-induced endothelial cell death and development of acellular capillaries in rat retina
doi: 10.1038/mtm.2015.13
Figure Lengend Snippet: Silencing p75 NTR suppresses proNGF-induced apoptotic markers in EC. Representative western blots and statistical analysis of ( a ) pJNK, total JNK, ( b ) pP38MAPK, total p38MAPK, and ( c ) cleaved-PARP (C-PARP), actin in EC lysates. 2 × 2 way analysis showed interaction between proNGF overexpression and silencing p75 NTR . Overexpression of proNGF induced EC apoptosis evident by activation of JNK, p38MAPK, and cleavage of PARP. These effects were blunted by silencing p75 NTR . Results presented as mean ± SD. n = 4–6 per group. * P < 0.05 versus other groups.
Article Snippet: For cell viability experiments,
Techniques: Western Blot, Over Expression, Activation Assay